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RNA and 2’-O-methyl RNA oligonucleotides
RNA and 2’-O-methyl RNA oligonucleotide synthesis is performed at
Gene Link using the b –cyanoethyl chemistry and state of the art synthesizers.
These involve proprietary software protocols with long coupling times and
specialized cycles to obtain ultra clean oligos.
RNA oligos are susceptible to degradation to the same extent as native RNA
extracted from various sources. An attractive alternate to prevent degradation
from nucleases is the use of 2’-O- methyl RNA bases, when specific 2’OH is
not required. The 2’-O- methyl oligonucleotides confer considerable nuclease
resistance and are similar in hydrogen bonding properties to RNA/RNA than the
lower RNA/DNA binding property. The coupling efficiency of 2’-O- methyl
phosphoramidite is also higher than the RNA monomers resulting in higher yield
of full length oligos.
Gene Link also offers custom synthesis of RNA and DNA chimeric oligos with
investigator specified ribo or deoxy bases or 2’-O-methyl bases. The chimeric
oligos can also be synthesized with the regular phoshodiester bonds or
substituted with phosphorothioate linkages. The combination of 2’-O- methyl
RNA bases with phosphorothioate internucleotide linkages imparts these oligos
greater nuclease resistance which is particularly useful for antisense studies
(please refer to our technical sheet on Antisense Oligonucleotides for other
modifications). Custom phosphorothioate oligonucleotides synthesized by Gene
Link can be specified to have all the diester bonds substituted or only some
selected diester linkages depending upon the researchers experimental
requirement. Substitution of all diester linkage is recommended to provide
greater nuclease resistance.
References
- Goodwin,J.T., Stanick,W.A. and Glick,G.D.
(1994) Improved solid phase synthesis of long oligoribonucleotides.
Application to tRNA (phe) and tRNA(gly). J. Org. Chem. 59:7941-7943
- Cotton,M., Oberhauser,B., Burnar, H. et al.
(1991) 2’O methyl and 2’O ethyl oligoribonucleotides as inhibitors of
the in vitro U7 snRNP-dependent messenger-RNA processing event. NAR
19:2629
- Milligan, J.F., Matteucci, M.D. and Martin,
J.C. (1993) Current concepts in antisense drug design. J. Medicinal
Chem. 36:1923-1937.
- Helene, C., Toulme, J. (1990) Specific
regulation of gene expression by antisense, sense and antigene nucleic
acids. Biochim. Biophys. Acta. 1049: 99-125.
- Weintraub, H. M. (1990) Antisense RNA and DNA.
Sci. Amer. 262:40-46.
- Iyer, R.P., Egan. W., Regan, J.B and Beaucage,
S.L. (1990) J. Am. Chem. Soc.112; 1253-1254.
- Wagner, R.W., Matteucci, M.D., Lewis, J.G., Gutierrez, A.J., Moulds, C.
and Froehler, B.C. (1993) Antisense gene inhibition by oligonucleotides
containing C-5 propyne pyrimidines. Science 260:1510-1513.
§ § § § § §
RNA Oligos
RNA synthesis is offered only
in the 1 umol scale with a minimum charge for a 20mer. In addition to
regular RNA bases, Gene Link offers 2’-O-methyl RNA bases and chimeric
DNA-RNA oligos. Turn around time is ~5 days.
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| RNA oligos*** |
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$20.00/base |
| 2’O methyl bases*** |
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$20.00/base |
| DNA-RNA chimerics, price per chimeric linkage |
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$75.00 |
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***minimum charges for 20mer
applies |
Custom Antisense Oligonucleotide Synthesis
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Synthesis Scale, $/base NO SET
UP CHARGES |
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Modification |
200 nmol |
1 m mol |
10 m mol |
15 m mol |
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Phosphorothioate |
$4.25 |
$6.50 |
$50.00 |
$55.00 |
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C-5 propyne dC (pdC) |
$130/3 sites
each additional site @
$25.00 |
$200/3 sites
each additional site @
$35.00 |
$600/3 sites
each additional site @
$100.00 |
$650/3 sites
each additional site @
$100.00 |
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C-5 propyne dU (pdU) |
$130/3 sites
each additional site @
$25.00 |
$200/3 sites
each additional site @
$35.00 |
$600/3 sites
each additional site @
$100.00 |
$650/3 sites
each additional site @
$100.00 |
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Purification*
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200
nmol
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1
m
mol
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10
m
mol
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15
m
mol
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Gel
Purification
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$75.00
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$150.00
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$1100.00
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$1300.00
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Reverse
Phase Cartridge
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$30.00
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$90.00
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$750.00
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$750.00
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*Desalted
and Lyophilized at no extra charge
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2'Omethyl.doc/revised
February 12, 1998
Prices subject to change without notice.All Gene Link products are for research use only.
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